Genomic SELEX: A discovery tool for genomic aptamers
نویسندگان
چکیده
منابع مشابه
Genomic SELEX for Hfq-binding RNAs identifies genomic aptamers predominantly in antisense transcripts
An unexpectedly high number of regulatory RNAs have been recently discovered that fine-tune the function of genes at all levels of expression. We employed Genomic SELEX, a method to identify protein-binding RNAs encoded in the genome, to search for further regulatory RNAs in Escherichia coli. We used the global regulator protein Hfq as bait, because it can interact with a large number of RNAs, ...
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An increasing number of proteins are being identified that regulate gene expression by binding specific nucleic acidsin vivo. A method termed genomic SELEX facilitates the rapid identification of networks of protein-nucleic acid interactions by identifying within the genomic sequences of an organism the highest affinity sites for any protein of the organism. As with its progenitor, SELEX of ran...
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Neptune locates genomic signatures using an exact k -mer matching strategy while accommodating k -mer mismatches. The software identifies sequences that are sufficiently represented within inclusion targets and sufficiently absent from exclusion targets. The signature discovery process is accomplished using probabilistic models instead of heuristic strategies. We have evaluated Neptune on Liste...
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Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps. RNA SELEX is more complicated than DNA SELEX because of the additional transcription and reverse transcription steps. Here, we report a new selection scheme, RAPID-SELEX (RNA Aptamer Isolation via Dual-cycles SELEX), that simplifies this...
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ژورنال
عنوان ژورنال: Methods
سال: 2010
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2010.06.004